Abbigael Eli
1 media/Abbigael Eli - can be a professional headshot_thumb.jpg 2020-05-05T22:35:24+00:00 Schmid College of Science and Technology ef61ed75d203ace65a2b05613a8adc7a45c04b00 18 1 B.S. Biochemistry & Molecular BiologyMinor: Rhetoric and Writing Studies
Mentor: Dr. Marco Bisoffi plain 2020-05-05T22:35:24+00:00 Schmid College of Science and Technology ef61ed75d203ace65a2b05613a8adc7a45c04b00
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2020-05-05T23:10:42+00:00
Abbigael Eli
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2020-05-08T23:41:13+00:00
Overview: Our research explores a potential drug for prostate cancer. The compound ca27, a synthetic analog of curcumin, limits the growth of prostate cancer cells by blocking androgen receptor (AR) function. This project studies the effect of ca27 on AR translocation to the cell nucleus as a potential mechanism of action.
CURE-Cumin: The Spice in Your Life Against Prostate Cancer
Abstract: Prostate cancer (PCa) is one of the most frequent cancers in the world’s male population. The androgen receptor (AR), which responds to the binding of androgens (for example, testosterone), is a major oncogenic driver in cancer cells. Androgens bind the AR in the cytoplasm and initiate its translocation to the nucleus, where it acts as a transcription factor for genes that promote growth and survival. In cancerous cells, AR signaling is upregulated and constitutive, leading to uncontrolled cell growth. The diarylpentanoid ca27, an analog of the natural product curcumin, has been shown to downregulate AR expression in PCa cells, but its mechanism of action remains unknown. This study explores the possibility of ca27 interfering with AR nuclear translocation, thereby leading to its degradation and reduced expression. Androgen-dependent human LNCaP prostate cancer cells were treated with ca27, curcumin, and dimethyl sulfoxide (DMSO) vehicle control, followed by the generation of cytoplasmic and nuclear protein lysates. The protein lysates were size-separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) followed by immunodetection of AR, histone H3 (nuclear protein), and beta-tubulin (cytoplasmic protein) by chemiluminescent Western blotting (WB). AR expression levels were determined by AR band densitometric analysis of digitalized images using ImageJ software. In this project, we aim at testing the potential effect of ca27 on AR translocation by determining the ratio of cytoplasmic-to-nuclear AR band intensity between ca27-, curcumin-, and DMSO-treated protein lysates. We report here on our first data set and provide an experimental plan moving forward to elucidate the effect of ca27 on AR translocation and expression as a potential mechanism of action.
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