Jordan Jernigan
1 media/Jordan Jernigan - IMG_2855_thumb.JPG 2020-05-05T22:44:26+00:00 Schmid College of Science and Technology ef61ed75d203ace65a2b05613a8adc7a45c04b00 18 1 B.S. Biochemistry & Molecular BiologyMentor: Dr. John Miklavcic plain 2020-05-05T22:44:26+00:00 154351 20200503 20200503 154351 33.901477777778,-118.37414444444 Schmid College of Science and Technology ef61ed75d203ace65a2b05613a8adc7a45c04b00
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2020-05-06T00:25:03+00:00
Jordan Jernigan
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Overview: Extracellular vesicles contain gangliosides. Dietary gangliosides are not considered essential fats and their health benefits remain unidentified. The purpose of this research was to characterize the change in total ganglioside content in human intestinal epithelial cells and EVs due to a treatment with dietary gangliosides.
Characterizing the Ganglioside Content in Caco-2 Human Intestinal Epithelial Cells and Extracellular Vesicles After Supplementation With Exogenous Gangliosides
Abstract: Dietary fats play a role in the development, progression, and treatment of chronic diseases. Gangliosides are a type of lipid, or fat, found in all human tissues. Both healthy and diseased cells undergo intercellular communication by secreting vesicles into the extracellular environment. Extracellular vesicles (EVs) contain many bioactive compounds, including gangliosides. Dietary gangliosides are not considered essential nutrients and their health benefits and therapeutic traits remain unidentified. The purpose of this research was to characterize the change in total ganglioside content in human intestinal epithelial cells and EVs due to a treatment with dietary gangliosides. Caco-2 human intestinal epithelial cells were given with an exogenous source of gangliosides (10 μg/mL of GM3 ganglioside or 10 μg/mL of GD3 ganglioside). Cell media was collected over 48 hr. EV and ganglioside isolation was complete. EV verification was achieved using immunoblotting and electron microscopy. A University of Alberta collaborator will complete lipidomic profiling on cells and EVs. It is anticipated that an exogenous source of gangliosides will alter the total ganglioside content and composition in cells and EVs. The findings of this study will support future research on the therapeutic applications of dietary gangliosides, as well as the physiochemical properties of dietary fats and EVs in relation to human chronic conditions.
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